Standardizing Cytometry Data Analysis

Standardization of flow cytometry assays requires careful attention to reagents, sample handling, instrument setup, and data analysis, and is essential for successful cross-study and cross-center performance and analysis of large data sets. The Human Immune Phenotyping Consortium (HIP-C) was formed by the Federation of Clinical Immunology Societies (FOCIS) to address these issues. While it is known that centralized manual gating can reduce cross-center variability, here we sought to determine whether automated methods were a suitable approach to streamline and further standardize analysis.

ImmunSpace Project Link

Related Publications

Standardizing Flow Cytometry Immunophenotyping Analysis from the Human ImmunoPhenotyping Consortium

PDF Code Project Project Source Document